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Preparation of casein phosphorylated peptides and casein non-phosphorylated peptides using alcalase

✍ Scribed by Li Zhao; Zhang Wang; Shi ying Xu


Publisher
Springer
Year
2006
Tongue
English
Weight
270 KB
Volume
225
Category
Article
ISSN
0044-3026

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Mass spectrometric characterization of p
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Matrix‐assisted laser desorption/ionization in‐source decay (MALDI‐ISD) has been used for characterization of a phosphorylated peptides and proteins because labile phosphate group is not lost during the MALDI‐ISD process. The conventional MALDI‐ISD is initiated by the hydrogen transfer from reducing