There is a large and increasing number of methods for preparing whole-cell DNA from fungi. Modifications have evolved for two reasons. This first is to simplify the protocol as much as possible to allow processing of large sample numbers, in some cases for very specific uses, e.g., dot-blots. The se
โฆ LIBER โฆ
Preparation of both DNA and RNA for hybridization analysis from limiting quantities of lymphoid cells
โ Scribed by Martin J. Pearse; Li Wu
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 346 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0165-2478
No coin nor oath required. For personal study only.
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## Background Current methods for accurate quantification of nucleic acids typically begin with a template preparation step in which DNA and/or RNA are freed of bound proteins and are then purified. Isolation of RNA is particularly challenging because this molecule is sensitive to elevated temperat