✦ LIBER ✦

Preparation of bacteriophage λ DNA using the TL-100 ultracentrifuge

✍ Scribed by Michael F. Buckley; James W. Goding

Book ID: 102986295
Publisher: Elsevier Science
Year: 1988
Tongue: English
Weight: 694 KB
Volume: 175
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(88)90389-2

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✦ Synopsis

A procedure for the preparation of DNA from bacteriophage X is described, using the Beckman TL-100 bench-top ultracentrifuge. The procedure involves growth of phage in agar plates, precipitation with polyethylene glycol, and a single centrifugation in cesium chloride under conditions that disrupt the phage coat. The method avoids the use of enzymes, ion exchange resins, and phenol. It can be completed in less than a day. The resulting DNA is of good purity and is easily cuttable by restriction enzymes. o 1988 Academic press, IIIC.

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