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Preparation of antibodies against hydrolyzable plant tannins

✍ Scribed by Daniela Kaspar; Petra Grundhöfer; Georg G. Gross

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
128 KB
Volume
9
Category
Article
ISSN
0958-0344

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✦ Synopsis

Polyclonal antibodies were raised against covalently protein-coupled 1,2,3,4,6-pentagalloylglucose, the common and immediate precursor of the two subclasses of hydrolyzable tannins, i.e. the polyphenolic gallotannins and ellagitannins. The crude antisera were efficiently purified by affinity-chromatography on pentagalloylglucose-substituted Sepharose. This antibody displayed strong affinity in heterogeneous competitive ELISAs towards both gallotannins and ellagitannins. In contrast, insignificant cross-reactivities, or none, were observed with dimeric proanthocyanidins (condensed tannins) and their monomeric precursors, as well as with low-molecular weight phenolics. The antibodies were inactive with aliphatic polyhydroxylated compounds, as has been shown for monomeric, dimeric and polymeric carbohydrates.

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