Preparation of a highly purified vaccine against tick-borne encephalitis by continuous flow zonal ultracentrifugation

✍ Scribed by Franz X. Heinz; Christian Kunz; Hans Fauma

• Publisher: John Wiley and Sons
• Year: 1980
• Tongue: English
• Weight: 645 KB
• Volume: 6
• Category: Article
• ISSN: 0146-6615
• DOI: 10.1002/jmv.1890060304

✦ Synopsis

Abstract

By the use of continuous‐flow zonal Ultracentrifugation, a highly purified, inactivated tick‐borne encephalitis (TBE) virus vaccine was prepared that contained almost exclusively TBE virus particles. The purity of this preparation was 90‐ to 100‐fold higher than that of a previously used partially purified vaccine. A mean protein content of 3–5 m̈g/ml in the final vaccine represents 20 mouse protective doses~50~ in a mouse challenge potency test. This high degree of purity resulted in a strong reduction of side reactions observed with the less purified vaccine.