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Preparation of 2-[125I] iodohistamine-labelled Δ8-tetrahydrocannabinol-11-oic acid for use in cannabinoid radioimmunoassay

✍ Scribed by B Law; P A Mason; A C Moffat; L J King


Publisher
John Wiley and Sons
Year
1982
Tongue
French
Weight
453 KB
Volume
19
Category
Article
ISSN
0022-2135

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✦ Synopsis


Abstract

A simple method is described for the preparation of 2‐[^125^I]iodohistamine‐labelled Δ^8^‐tetrahydrocannabinol‐11‐oic acid with high specific activity for use in radioimmunoassay. This compound is produced in high yield and shows excellent radiochemical stability when stored at 4°C. The radiolabelled cannabinoid has been shown to bind avidly to four different broadly specific cannabinoid antisera. It is the only [^125^I] labelled cannabinoid suitable for use in radioimmunoassay with such antisera.