Preparation of 14C-labelled AMP, ADP AND ATP from adenine-8-14C by using brevibacterium ammoniagenes
✍ Scribed by V. N. Pande
- Publisher
- John Wiley and Sons
- Year
- 1985
- Tongue
- French
- Weight
- 176 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0022-2135
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✦ Synopsis
High radiochevdcal y i e l d s of 1 4 ~l a b d l . d admine rucleotider, (AW, 4.6%; AIW, 15.5% and .UP, 59.9%) could be obtained by gmrrlng t h e cells of & w l b a c t e r ~ gmmn iaaene i n t h e presence of l h d m i n e . Tho s p e c i f i c radioactivity of : he adenlne t u c 1 ~t l d e s almb+t n r 0 h . d t h a t of 1 h d m i n indicating ntgliglble d i l u t i o n of t h e labol. The procedure is eanvcnient and especially r u l t d for cannorela1 proparation of t h e radiolabelled n u c l w t i d e s di&W fmm labelled admine. P r o l i d n a r y r e u l t r indicate t h a t t h e organism could also be card for the pmprratlon of rrdlolabelled qwnine nucleotldm. Adenine mclmtld@ labelled d t h 14C-radioisotope a r e useful conpaunds for hioch.mlca1 research. T h rdloactive ruclootides a r e expensive since t h e COfNnmCi81 p r o c s b r e s for t h e preprration of these involve either 8 rmltistep chemlcal synthesis or 8n mzyraatic r y n t h s i s using a nrmber of purified enrymrs and l r b l l d prea~r@on. The present comunication d e s c r i b e a sinple and inexpensive p n x u h r e for the preparation of a d e r ~r i r u -8 -~~C mno-, dl-, and trlphosphste of h i g h mpecific radioactivity f r o m ddenlne-8-14C by a o d n g c d l r of m n i a a e n a . Thin bacterium is know t o accumlate large amuntr of adanina nuclootlder i n the nsdlua when grow d t h admine (1, 2). A typlcal procdurr used for the preparation of labelled adenine nucl.ot1d.s using t h i s bacterium 10 described 68 fol&rC: Br.Vibra m n o n i a m s ( A T E 6B72) ms mintained on mtrient agar rlmts. The bacterium MB prom