Preparation of 14C- and 3H-methoxyl-labelled forms of ubiquinone by photochemical O-demethylation and subsequent remethylation
✍ Scribed by Hong-Ming Cheng; John E. Casida
- Publisher
- John Wiley and Sons
- Year
- 1970
- Tongue
- French
- Weight
- 588 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0022-2135
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✦ Synopsis
Ubiquinone [ubiquinone (50), coenzyme Q16, CoQ,,] undergoes 0-demethylation to the 2-and 3-hydroxyubiquinones, reduction to the hydroubiquinone, and cyclization to the ubichromenol derivative when irradiated, in alcohol solution, with a sunlamp. It is likely that the ubichromenol and hydroubiquinone derivatives form via a common intermediate which is diyerent from that involved in the formation of the hydroxyubiquinone derivatives. The mixture of hydroxyubiquinones, on methylation with 14C-and 3H-labelled methyl iodide or diazomethane, yields rnethoxyl-labelled ubiquinone, a material useful in the study of ubiquinone photodecomposition, metabolism, and biochemistry.