Preparation of [11C]-thymidine and [11C]-2′-arabino-2′-fluoro-β-5-methyl-uridine (FMAU) using a hollow fiber membrane bioreactor system
✍ Scribed by Jeffrey A. Hughes; Neil G. Hartman; Michael Jay
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- French
- Weight
- 531 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0022-2135
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✦ Synopsis
Abstract
A series of hollow fiber membranes containing immobilized enzymes were prepared and used in the synthesis of ^11^C‐labelled nucleosides. ^11^C‐Formaldehyde was produced in an alcohol oxidase/catalase bioreactor and circulated through a thymidylate synthase bioreactor with an appropriate substrate to produce the corresponding ^11^C‐nucleotide. These labelled nucleotides were subsequently dephosphorylated in an alkaline phosphatase bioreactor. The bioreactor approach was amenable to hot‐cell conditions and yielded ^11^C‐products in higher yield and shorter synthesis times than conventional chemical approaches.