Preparation in undenatured form of the main protein bound to heterogeneous nuclear RNA in liver and hepatoma cells

✍ Scribed by A. Schweiger; G. Kostka

• Book ID: 104768635
• Publisher: Springer
• Year: 1977
• Tongue: English
• Weight: 458 KB
• Volume: 3
• Category: Article
• ISSN: 0301-4851
• DOI: 10.1007/bf00420394

✦ Synopsis

Particles carrying heterogeneous nuclear RNA (30 S-particles) were prepared from rat liver and Zajdela hepatoma ascites cell nuclei after ultrasonic disruption. The ribonucleoprotein structures were disintegrated in the presence of 100mM spermidine. Using chromatography on Sepharose-polyadenylate a protein component has been obtained which possessed high affinity for heterogeneous nuclear RNA, polyuridylate and polyadenylate, and double-stranded DNA. This protein was the main species of the ribonucleoprotein studied; it showed bands with molcular weights of 37000 and 40000 respectively in SDS gel electrophoresis. The RNA-binding proteins isolated from liver and hepatoma had identical molecular weights and the same affinity for Sepharose-polyadenylate used in the isolation.