Preparation, characterization and cytocompatibility of porous ACP/PLLA composites

✍ Scribed by Yanbo Gao; Wenjian Weng; Kui Cheng; Piyi Du; Ge Shen; Gaorong Han; Binggang Guan; Weiqi Yan

• Publisher: John Wiley and Sons
• Year: 2006
• Tongue: English
• Weight: 582 KB
• Volume: 79A
• Category: Article
• ISSN: 1549-3296
• DOI: 10.1002/jbm.a.30761

✦ Synopsis

Abstract

The purpose of this work was to incorporate amorphous calcium phosphate (ACP) into porous poly(L‐lactic acid) (PLLA), because ACP is capable of fast phase transformation and morphological change in body fluid, such, a desired pore wall surface within bone tissue engineering scaffolds can be created. A highly porous ACP/PLLA composite was prepared by a thermally induced phase separation technique. The results showed that the composite had an interconnected pore structure with 100 μm macropores and 10 μm micropores, and 91% porosity; 40 nm primary particles of ACP were agglomerated to 3 μm aggregates, and the aggregates were homogeneously distributed in pore walls; These aggregates showed to be in situ transformed into bone‐like apatite after 1 h soaking in phosphate buffered saline solution. Human osteoblast‐like cell culture showed that the ACP/PLLA composite had better cell adhesion and alkaline phosphotase activity than pure PLLA. This study demonstrates that the ACP/PLLA composite can enhance cytocompatibility and could act as a promising scaffold for bone tissue engineering. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006