Preparation, characterization and application of a new 25,27-bis-[2-(5-methylthiadiazole)thioethoxyl]-26,28-dihydroxy-para-tert-butyl calix[4]arene stationary phase for HPLC
✍ Scribed by Kai Hu; Junwei Liu; Chao Tang; Caijuan Wang; Ajuan Yu; Fuyong Wen; Wenjie Zhao; Baoxian Ye; Yangjie Wu; Shusheng Zhang
- Publisher
- John Wiley and Sons
- Year
- 2012
- Tongue
- English
- Weight
- 288 KB
- Volume
- 35
- Category
- Article
- ISSN
- 1615-9306
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✦ Synopsis
Abstract
A new para‐tert‐butylcalix[4]arene column containing thiadiazole functional groups was prepared and used for the separation of polycyclic aromatic hydrocarbons, phenolic compounds, aromatic amines, benzoic acid and its derivatives by high‐performance liquid chromatography (HPLC). The effect of organic modifier content in the mobile phase on retention and selectivity of these compounds were investigated. The results indicate that the stationary phase behaves like reversed‐phase packing. However, hydrogen bonding, π–π and inclusion interactions seem to be involved in the separation process. The column has been successfully employed for the analysis of clenbuterol in pork and pig casing; the limit of detection and the limit of quantitation for this method by HPLC‐UV detection was 0.03 and 0.097 μg/mL, respectively; the method is demonstrated to be suitable and a competitive alternative analytical method for the determination of clenbuterol.