Preparation, characterization, and antimicrobial activity of quaternized chitosan/organic montmorillonite nanocomposites

✍ Scribed by Xiaoying Wang; Yumin Du; Jianhong Yang; Yufeng Tang; Jiwen Luo

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 199 KB
• Volume: 84A
• Category: Article
• ISSN: 1549-3296
• DOI: 10.1002/jbm.a.31326

✦ Synopsis

Abstract

Quaternized chitosan/layered silicate nanocomposite was prepared by simple solution‐mixing in aqueous media. Montmorillonite (MMT) modified with cetyltrimethyl ammonium bromide was used as an organically modified layered silicate. XRD and TEM analyses respectively confirmed that silicate layers of MMT were intercalated and nicely distributed in quaternized chitosan matrix in despite of the high content of MMT (25–50 wt %). The interactions between the quaternized chitosan macromolecules and MMT in aqueous media were analyzed using FTIR, XRD, and ζ‐potential measurements. Antimicrobial studies showed that the nanocomposites could strongly inhibit the growth of a wide variety of microorganisms, including Gram‐positive bacteria, Gram‐negative bacteria, and fungi; more importantly, they exhibited good antimicrobial capacity in whichever medium, in weak acid, water, or weak base. As the amount of MMT increased, the nanocomposites had better inhibitory effect on microorganisms, especially Gram‐positive bacteria. The lowest minimum inhibition concentration (MIC) value of the nanocomposites against Staphylococcus aureus and Bacillus subtilis were less than 0.00313% (w/v) under all the conditions. The adsorption action of MMT on bacteria was simply discussed via SEM images. The results revealed that the strong antimicrobial of the nanocomposites may be attributed to the fine dispersion and the interaction between quaternized chitosan and MMT. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008