β Scribed by Dr. E. Robert Burns; C. Bruce Bagwell; William G. Hinson; James L. Pipkin Jr.; Jerry L. Hudson
No coin nor oath required. For personal study only.
Three different technical protocols were used to prepare samples for flow cytometric (FCM) analysis. Each protocol developed worked best for only certain organs. Protocol I involved mincing small pieces of fresh tissue in the propidium iodide (PI) staining solution and filtering through packed glass wool. The organs that were prepared by protocol I were: submandibular gland, urinary bladder, liver, thymus, bone marrow, spleen, lung, kidney and testis. Protocol I1 involved exposure of the organ to 0.5% acetic acid for 48 h prior to mincing in the PI. The organs that were prepared by protocol I1 were: uterus, rectum, colon, ileum, and heart. Protocol I11 utilized a n exposure to 0.5% acetic acid, pepsinization, and then staining with PI. The tissues 'J.L. Hudson's present address is
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Routine flow cytometric DNA analysis was compared in two laboratories by using matched fresh-frozen breast cancer and soft tissue sarcoma biopsy specimens. Laboratory I applied the VindelΓΆv preparation method and an exponential background subtraction algorithm in the cell cycle calculation. Laborato
A modification of the Hedley-method for flow cytometric DNA analysis of paraffin-embedded tissues is presented. Dewaxed and dehydrated tissue from paraffin blocks was incubated with subtilisin Carlsberg (pronase, Sigma protease XXIV) and then stained directly without washing and centrifugation. The
## BACKGROUND. Confusing data have been presented for breast cancer patients on correlations between DNA ploidy and the percentage of S-phase cells and other prognostic variables. The aim of this study was to compare DNA ploidy classification and cell cycle variables with clinical, classic, and qua