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Preparation and separation properties of open tubular columns with immobilized Carbowax 20M for LC

✍ Scribed by Karel Janák; Marie Horká; Miloš Krejčí

Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
441 KB
Volume
3
Category
Article
ISSN
1040-7685

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✦ Synopsis

Abstract

Open tubular columns (3‐5 m × 23‐50 μm i.d.) were coated with Carbowax 20M (0.07‐0.2 μm film thickness) and immobilized with pluriisocyanates (Desmodur N or Desmodur L), a method which is simple, rapid, and reproducible. The concentration of cross‐linking agent and the preparation conditions were optimized with respect to stability and separation properties of the immobilized film under liquid chromatography (LC) conditions. Properties of columns in LC systems are strongly dependent on the degree of immobilization and slightly dependent on the type of Desmodur used as a cross‐linking agent. The degree of immobilization must exceed 90% for successful application in LC. Such columns displayed good long‐term stability, especially when immobilized with Desmodur L. Using water as the mobile phase, the LC system behaved as a reversed phase system. Capacity ratio and temperature strongly affected the separation efficiency.

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