Preparation and properties of nano-hydroxyapatite/PCL-PEG-PCL composite membranes for tissue engineering applications
✍ Scribed by Shao Zhi Fu; Xiu Hong Wang; Gang Guo; Shuai Shi; Min Fan; Hang Liang; Feng Luo; Zhi Yong Qian
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 768 KB
- Volume
- 97B
- Category
- Article
- ISSN
- 1552-4973
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Nano‐hydroxyapatite (n‐HA)/poly(ε‐caprolactone)‐poly(ethylene glycol)‐poly(ε–caprolactone) (PCL‐PEG‐PCL, PCEC) composite membranes were prepared by solvent casting and evaporation method. The structure and properties of the membranes were investigated by Fourier transform infrared spectroscopy (FT‐IR), X‐ray diffraction (XRD), scanning electron microscopy (SEM), water contact angle measurements, in vitro hydrolytic degradation, mechanical test, and cell culture. The effect of n‐HA content on physical–chemical properties of the n‐HA/PCEC composite membranes was studied. The results showed that the shape and size of micropores of the composite membranes changed with n‐HA content increased; the tensile strength decreased with the increase of n‐HA content. The osteoblast cell was cultured on the membranes, good cell attachment and growth manner were observed after postseeding for 1 day. MTT assays showed that the n‐HA/PCEC membranes had no negative effect on the cell viability and proliferation. These results suggested that the obtained n‐HA/PCEC composite membranes in this study might have prospective applications in tissue engineering field. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2011.