Preparation and properties of metabolically 3H- or 13C-labeled (1 → 3)-β-d-glucan, SSG, from Sclerotinia sclerotiorum IFO 9395

✍ Scribed by Masahiro Suda; Naohito Ohno; Yoshiyuki Adachi; Toshiro Yadomae

• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 491 KB
• Volume: 254
• Category: Article
• ISSN: 0008-6215
• DOI: 10.1016/0008-6215(94)84254-x

✦ Synopsis

Metabolically labeled (1 + 3)-p-o-glucan (SSG) obtained from the culture filtrate of Sclerotinia sclerotionun IF0 9395, with the radio c3H) or the stable isotope (r3C) was prepared. The specific radioactivity of 'H-SSG was increased in accordance with the amount of o-[3H]glucose added, and was 15-20 kBq/mg SSG when 222 kBq/mL of o-[3H]gluUXe was added. Physicochemical analyses of 3H-SSG suggested a structural similarity between SSG and 3H-SSG. 13C NMR spectra of 13C-labeled SSG (r3C-SSG) revealed that a strong signal of the t3C-nucleus was observed at the C-l or C-2 position when D-[1-'3C]glucose or o-[2-'%&lucose was used, respectively, suggesting that most of the glucose residues in SSG were directly taken up from the medium. In addition, part of the t3C-nucleus was incorporated into the SSG molecule at all carbon atoms after metabolic degradation and reconstruction of the glucose molecule. Analyses of the culture filtrate and the mycelium of the fungus suggested that part of the glucose was also metabolized to trehalose and mannitol.