Preparation and properties of a cysteine analog of human insulin
β Scribed by Yu. P. Shvachkin; S. M. Funtova; A. M. Nikitina; S. P. Krasnoshchekova; T. M. Anokhina; V. P. Fedotov; A. I. Ivanova
- Publisher
- Springer
- Year
- 1986
- Tongue
- English
- Weight
- 144 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0009-3130
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π SIMILAR VOLUMES
## Abstract Malonate diesters containing a prochiral quaternary carbon have been successfully transformed into analogs of cysteine and serine. The chiral halfβesters are obtained in good yield, and enantioselectivity by selective hydrolysis using PigβLiver Esterase (PLE) as the catalyst. The result
The trypsin-catalyzed coupling of bovine (Boc)z-desoctapeptide (B23-B30)-insulin with synthetic octapeptides, H-Gly-Xz-X3-X4-Thr-Pro-Lys(Boc)-Thr-OH (Xz = Phe or Ala, X3 = Phe or Ala, X4 = Tyr or Ala), followed by deprotection and purification produced the [AlaBZ4, ThrB30]-, [AlaBZ5, ThrBN] -, and [