Preparation and PCR-Amplification Properties of a Novel Amphiphilic Poly(N-vinylpyrrolidone) (PVP) Copolymer

✍ Scribed by Lifen Zhang; Yong Liang; Lingzhi Meng; Xiaoju Lu; Yunhai Liu

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 286 KB
• Volume: 4
• Category: Article
• ISSN: 1612-1872
• DOI: 10.1002/cbdv.200790021

✦ Synopsis

Abstract

A novel amphiphilic copolymer, P(NVP‐co‐TrpAMT) (9) was prepared, comprising hydrophilic N‐vinylpyrrolidone (NVP; 8) and hydrophobic ‘N‐[(tert‐butoxy)carbonyl]tryptophanamido‐N′‐methacryl thiourea’ (TrpAMT; 7) segments. The amphiphilic copolymer 9 was characterized by ^1^H‐NMR, GPC‐MALLS, TEM, and MTT assay. It has a critical micelle concentration (cmc) of 45.7 mg/l in aqueous solution, and good biocompatibility in vivo. According to TEM, the polymer is mostly present as spherical micelles in water, with a diameter of ca. 60–90 nm. In the presence of 0.1 μg/ml of 9, the PCR amplification of the GC‐rich β‐actin was efficiently enhanced. Also, the fluorescence intensity of the reporter dye SYBR Green I was increased by 26% at the 14th cycle during real‐time PCR of plasmid pUC18 DNA.