Preparation and Fluorescence Characteristics of Amido-Functionalized Dual-Fluorescent Microspheres with Core/Shell Structure
✍ Scribed by Xinghua Pan; Jianhui Ju; Yonghua Zhan; Daocheng Wu
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 550 KB
- Volume
- 211
- Category
- Article
- ISSN
- 1022-1352
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✦ Synopsis
Abstract
A novel two‐step modified seeded polymerization method for the preparation of AFDFMs with a core/shell structure is developed. Their morphology is characterized by means of TEM and SEM, further characteristics are studied using FT‐IR, fluorescence microscopy, laser confocal fluorescence microscopy, and fluorescence spectroscopy. TEM and SEM images provide clear proof of a core/shell structure. Fluorescence spectroscopy shows that AFDFMs exhibit tunable fluorescence characteristics with one or two emission wavelengths (515 and 575 nm) depending on the excitation wavelengths. Amido and carboxy groups on the AFDFM shell layer allow the formation of linkages with biomolecules for biological imaging or molecule detection, opening opportunities for biomedical applications. magnified image
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