Preparation and evaluation of an IgG-Lucifer Yellow carbohydrazide conjugate for the detection of measles antigen

✍ Scribed by M. Njayou; C. Mouliom

• Book ID: 104739070
• Publisher: Springer
• Year: 1996
• Tongue: English
• Weight: 174 KB
• Volume: 12
• Category: Article
• ISSN: 1573-0972
• DOI: 10.1007/bf00360932

✦ Synopsis

A conjugate, prepared by covalently coupling Lucifer Yellow carbohydrazide (LYCH) to monkey anti-measles IgG, was used to detect measles antigen in infected cell monolayers and in nasopharyngeal swabs from children. Compared with fluorescein isothiocyanate, LYCH is not only easier to couple to antibodies but also produces better fluorescence.