Preparation and characterization of plasmid DNA network via both triple helix formation and photo-crosslinking

✍ Scribed by Yosuke Kawabata; Tooru Ooya; Nobuhiko Yui

• Publisher: Institute of Physics and National Institute of Materials Science
• Year: 2003
• Tongue: English
• Weight: 151 KB
• Volume: 4
• Category: Article
• ISSN: 1468-6996
• DOI: 10.1016/s1468-6996(03)00020-2

✦ Synopsis

New self-assembled network of plasmid DNA was prepared via both photo-crosslinking and triple helix formations, and evaluated the conformation of DNA network by electrophoresis. Two kinds of TFO sequences were designed to form the triple helix with pUC19 at different positions. In addition to the triple helix formation, a photo-crosslinking and lectin-molecular recognition were applied to form pUC19 network. Psoralen, photoreagent, and biotin were attached to 5 0 -and 3 0 -ends of the TFO, respectively. The biotin-TFO -psoralen conjugate, pUC19, and SA were mixed in a buffer and stand at 4 8C. The results of electrophoresis study indicated that both triple helix formation and photo-crosslinking with pUC19 were necessary for the network formation. Therefore, this method can provide stable plasmid DNA network that may be useful to manipulate nanostructure using typical plasmid DNA conformational changes: supercoiled circular and open circular forms.