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Preparation and characterization of monoclonal antibodies which recognise different gibberellin epitopes

โœ Scribed by J. P. Knox; M. H. Beale; G. W. Butcher; J. MacMillan


Publisher
Springer-Verlag
Year
1987
Tongue
English
Weight
540 KB
Volume
170
Category
Article
ISSN
0032-0935

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โœฆ Synopsis


The production and characterization of high-affinity monoclonal antibodies (McAb) to gibberellins (GAs) is reported. Hybrid myelomas were derived from immuuisations with conjugates in which immunogenic proteins were linked to GAI at carbon-3 and to GA 4 and GA9 at carbon-17. A series of McAb which display specificities allowing recognition of, and the discrimination between GA1, GA20, GA4 and GA9 is described. These McAb can be used in quantitative immunoassays for underivatised GAs.


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