Preparation and characterization of high-molecular-weight sericin by γ irradiation

✍ Scribed by Nak-Yun Sung; Eui-Baek Byun; Sun-Kyu Kwon; Young-Choon Yoo; Pil-Mun Jung; Jae-Hun Kim; Jong-Il Choi; Mee-Ree Kim; Ju-Woon Lee

• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 518 KB
• Volume: 120
• Category: Article
• ISSN: 0021-8995
• DOI: 10.1002/app.33332

✦ Synopsis

We conducted this study to examine the changes in the molecular structure and physiological activities of silk sericin after c irradiation. Sericin from Bombyx mori was extracted with an Na 2 CO 3 solution. The molecular weight distribution of sericin increased in the gel permeation chromatography and sodium dodecyl sulfate/polyacrylamide gel electrophoresis results as the irradiation dose increased. Circular dichroism data also revealed that the a-helix contents decreased with the irradiation dose. Ultraviolet absorption was shown a different pattern between the irradiated and unirradiated sericin. However, the Fourier transform infrared spectrum was not changed in all of the groups. Furthermore, the irradiated sericin was significantly increased in 2,2-diphenyl-1-picryl-hydrazil radical scavenging, and the tyrosinase inhibitory activities increased with irradiation dose. Therefore, c irradiation was an effective method for producing high-molecular-weight sericin and for developing functional foods and cosmetics.