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Preparation and Characterization of Biodegradable Microspheres Containing Hepatitis B Surface Antigen

✍ Scribed by Shaobing Zhou; Xueyan Liao; Zhenglun Liang; Xiaohong Li; Xianmo Deng; Heming Li

Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
161 KB
Volume
4
Category
Article
ISSN
1616-5187

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✦ Synopsis

Abstract

Summary: Poly‐DL‐lactide‐poly(ethylene glycol) (PELA) microspheres containing Hepatitis B surface antigen (HBsAg) were elaborated by a solvent extraction method based on the formation of a double water/oil/water (w/o/w) emulsion. Microspheres were characterized in terms of morphology, size and size distribution, encapsulation efficiency, and the efficiency of microsphere formation (EMF). Transmission electron microscopy (TEM) and polyacrylamide gel electrophoresis (PAGE) were used to investigate the structural integrality of HBsAg encapsulated in PELA microspheres. The release profile was investigated by the measurement of antigen present in the release medium at various intervals. The PELA‐10 microspheres displayed the highest antigen encapsulation efficiency (about 80%), and antigen molecules could be stabilized in the PELA‐10 microspheres during the preparation process. It suggested that the PELA microspheres had a great potential as a new polymer adjuvant for HBsAg.

The release of Hepatitis B surface antigen from poly‐DL‐lactide‐poly(ethylene glycol) microspheres.

imageThe release of Hepatitis B surface antigen from poly‐DL‐lactide‐poly(ethylene glycol) microspheres.

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