Preparation and biodistribution of 32P-labelled hydroxocobalamin and comparison with 57Co-labelled cobinamide and cyanocobalamin
✍ Scribed by Ralph Gräsbeck; Mirkka Sarparanta
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- French
- Weight
- 233 KB
- Volume
- 51
- Category
- Article
- ISSN
- 0022-2135
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✦ Synopsis
Abstract
To study whether in biodistribution studies the phosphorus‐containing side‐chain of cobalamin and the central cobalt atom behave differently, ^32^P‐ and ^57^Co‐labelled hydroxocobalamins were biosynthesized. A considerably higher specific activity of the ^32^P‐labelled compound was achieved than before. Both labelled hydroxocobalamins were administered in physiological quantities subcutaneously and orally to rats. The distributions of the two isotopes did not differ significantly. To explain previously observed slight deviations from this pattern, radiolabelled dicyano cobinamide and cyanocobalamin were prepared and studied in an analogous manner. Generally they behaved as hydroxocobalamin, but with greater accumulation in the liver, possibly reflecting the cyanide‐detoxifying function of this organ. Copyright © 2008 John Wiley & Sons, Ltd.