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Preparation and application of a partially degradable gel in mass spectrometry-based proteomic analysis

✍ Scribed by Jian Zhou; Yongbo Hu; Yong Lin; Haipeng Liu; Peng Xie


Book ID
116620525
Publisher
Elsevier Science
Year
2011
Tongue
English
Weight
565 KB
Volume
879
Category
Article
ISSN
1873-376X

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Separation and relative quantitation of complex protein mixtures remain two of the most challenging aspects of proteomics. Here an advanced technique called fluorescence difference 2-D gel electrophoresis technology (2D-DIGE) has been applied to a model system study of the Escherichia coli proteome