Prenatal TCDD causes persistent modulation of the postnatal immune response, and exacerbates inflammatory disease, in 36-week-old lupus-like autoimmune SNF1 mice
✍ Scribed by Amjad Mustafa; Steven Holladay; Sharon Witonsky; Kurt Zimmerman; Ashley Manari; Sheryl Countermarsh; Ebru Karpuzoglu; Robert Gogal
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 386 KB
- Volume
- 92
- Category
- Article
- ISSN
- 1542-9733
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✦ Synopsis
BACKGROUND: Prenatal exposure to the persistent environmental pollutant and model Ah receptor agonist, 2,3,7,8tetrachlorodibenzo-p-dioxin (TCDD), has been shown to permanently suppress postnatal cell-mediated immunity. More recently, skewing of select adult T and B cell responses toward enhanced inflammation has also been described in C57BL/6 mice after prenatal TCDD. This raises questions about adverse postnatal immune consequences of prenatal TCDD in animals genetically predisposed to inappropriate inflammatory responses. METHODS: Lupus-prone SNF 1 mice were exposed to 0, 40, or 80 mg/kg TCDD on gestation day (gd) 12 and examined at 36 weeks-of-age for immunomodulatory effects that correlated with worsened lupus pathology. RESULTS: Bone marrow pro-and large pre-B cells were decreased by prenatal TCDD, in both adult male and female mice, as were pre-and immature B cells. Splenic CD23 À CD1 hi and CD19 1 CD5 1 B cells were increased in males, as were B220 hi B cells in females, further suggesting persistent disruption of B cell lymphopoiesis by prenatal TCDD. Female mice displayed decreased IL-10 production by ConA-activated splenocytes, while males underproduced IL-4. Autoreactive CD4 1 Vb17a 1 spleen T cells were increased in both sexes by 80 mg/kg TCDD. Male mice but not females showed increased anti-ds DNA and cardiolipin autoantibody levels. CONCLUSIONS: Prenatal TCDD augmented the hallmark indicators of SLE progression in the lupus-prone SNF 1 mice, including renal immune complex deposition, glomerulonephritis, and mesangial proliferation. Prenatal TCDD therefore caused persistent modulation of the postnatal immune response, and exacerbated inflammatory disease, in lupus-like autoimmune SNF 1 mice. Birth Defects Res (Part B) 92: 82-94, 2011.