Prenatal detection of chromosome disorders
β Scribed by Kathy Mann; Caroline Mackie Ogilvie
- Book ID
- 117278936
- Publisher
- The Lancet
- Year
- 2001
- Tongue
- English
- Weight
- 45 KB
- Volume
- 358
- Category
- Article
- ISSN
- 0140-6736
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Specific amplification of a DNA segment of the human ZFY gene by polymerase chain reaction (PCR) was carried out for detection of Y-chromosome specificsequences. When male DNA was used as template for amplification, a single and discrete 530 bp ethidium bromide staining band was observed in agarose
A 13q isodisomy in a balanced karyotype: 45,XY,-13,-13,+i(13)(q10) was found in cultured amniocytes studied because of advanced maternal age. The isochromosome was monocentric and a new mutation as both parents had normal chromosomes. Fetal blood was studied to exclude 13-trisomy mosaicism. All (100