Preliminary β-tricalcium phosphate coating prepared by discharging in a modified body fluid enhances collagen immobilization onto titanium
✍ Scribed by Mizuyo Hosaka; Yo Shibata; Takashi Miyazaki
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 289 KB
- Volume
- 78B
- Category
- Article
- ISSN
- 1552-4973
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✦ Synopsis
Abstract
Because of its excellent scaffold properties toward bone cells, collagen has been recognized as a promising extracellular matrix protein for surface modification of titanium implants. Hydroxyapatite (HA) coatings have been investigated as a preliminary coating for collagen immobilization on titanium implants. However, the composition of HA‐collagen is recognized as being difficult, and while many studies have suggested that biodegradable β‐tricalcium phosphate (β‐TCP) has better osteoconductivity than HA, the efficiency of preliminary β‐TCP coating for collagen immobilization on titanium surfaces has yet to be evaluated. This investigation aimed to evaluate the applicability of HA and β‐TCP coatings, prepared by discharging in modified body fluids, as preliminary collagen coatings. To increase collagen induction on preliminary HA and β‐TCP coatings, we used a new cathodic polarization method. X‐ray photoelectron spectroscopy revealed that the bonding strength between the collagen NH^+^ amino groups of collagen and phosphate (PO~4~^3−^) was greater on the β‐TCP coating than the HA coating. The preliminary β‐TCP coating was tightly crosslinked with RCOO^−^ carboxyl groups of the collagen molecules and showed high cellular responses, even in the early stage of cell cultivation. Thus, this coating was found to be more effective than HA as a preliminary coating for collagen immobilization on titanium implants. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2006