Preliminary investigations on the immobilization of yeast alcohol dehydrogenase
β Scribed by N. Kelly; A. Flynn; D. B. Johnson
- Publisher
- John Wiley and Sons
- Year
- 1977
- Tongue
- English
- Weight
- 142 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0006-3592
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π SIMILAR VOLUMES
Four new symmetrical colourless biomimetics bearing two monochlorotriazine rings have been rationally designed and synthesized. These immobilized colourless ligands and one analogue of Cibacron Blue F3GA on Sepharose CL-4B were used to purify alcohol dehydrogenase from baker's yeast extract. Twenty-
## Abstract Alcohol dehydrogenase has been immobilized to the basic copolymer and its several derivatives using various techniques. Enzyme coupling to the supports with amino groups by means of glutaraldehyde was found the most suitable. Activity of alcohol dehydrogenase coupled to these amino supp