Preliminary investigations of an amperometric oligosaccharide dehydrogenase-based electrode for the detection of glucose and some other low molecular weight saccharides

✍ Scribed by T. Ruzgas; E. Csöregi; I. Katakis; G. Kenausis; L. Gorton

• Book ID: 102659479
• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 539 KB
• Volume: 9
• Category: Article
• ISSN: 0952-3499
• DOI: 10.1002/(sici)1099-1352(199634/12)9:5/6<480::aid-jmr287>3.0.co;2-8

✦ Synopsis

Biosensors for the determination of sugars were constructed using oligosaccharide dehydrogenase (ODH) and soluble phenazine methosulfate (PMS) or an osmium-based three-dimensional redox hydrogel. In the latter case the enzyme and poly( 1-vinylidazole) complexed with osmium (4,4'-dimethylbpy),CI were cross-linked with poly(ethy1ene glycol) diglycidyl ether. Both electrode configurations showed similar sensitivities for glucose in the range between 8 and 21 pAmM-' ern-*. The responses for 10 mono and oligosaccharides were studied. There was no response for fructose. In the concentration range 0.1-20 m~ the relative sensitivities were determined for arabinose (96%), xylose (3%), mannose (50%), galactose (11%), glucose (loo%), maltose (%%), lactose (12%), cellobiose (34%) and maltotriose (10%).