Preliminary crystallographic analysis of class 3 rat liver aldehyde dehydrogenase

✍ Scribed by John P. Rose; John Hempel; Ingrid Kuo; Ronald Lindahl; Professor Bi-Cheng Wang

• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 341 KB
• Volume: 8
• Category: Article
• ISSN: 0887-3585
• DOI: 10.1002/prot.340080404

✦ Synopsis

Abstract

NAD‐linked aldehyde dehydrogenases (A1DH) (EC 1.2.1.3) catalyze the irreversible oxidation of a wide variety of aldehydes to their respective carboxylic acids. Crystals of a class 3 A1DH (from an Escherichia coli expression system) suitable for X‐ray analysis have been obtained. These crystals, which can be grown to a size of 0.8 × 0.3 × 0.2 mm, diffract to 2.5 Å resolution. Analysis of the diffraction pattern indicates that the crystals belong to the monoclinic space group __P__2~1~, with cell parameters a = 65.11 Å, b = 170.67 Å, c = 47.15 Å, and β = 110.5°. Assuming one dimer per asymmetric unit, the value V~m~ is calculated to be 2.45 and the solvent content of the crystal is estimated to be 50%. A self‐rotation function study produced significant rotation peaks (58% of the origin) on the K = 180 section at ψ = 90° and ϕ = 71° and 341°, indicating that the pseudo‐dimer axis is (or is very nearly) perpendicular to the b‐axis.