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Preliminary characterization of a mutant of herpes simplex virus type 1 selected for acycloguanosine resistance in vitro

✍ Scribed by Dr. Giorgio Palú; Wilma P. Summers; Sebastiano Valisena; Mauro Tognon

Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
708 KB
Volume
24
Category
Article
ISSN
0146-6615

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✦ Synopsis

In this paper we report on the preliminary characterization of a mutant of herpes simplex virus type. 1 (HSV-1) selected for acycloguanosine (acyclovir, ACV) resistance in vitro. The ACV' virus was examined for a series of parameters that include chemosensitivity assay, thymidine kinase (TK) activity, in vitro and in vivo growth, and mutation mapping. The data obtained indicate that a mutated TK gene is responsible for the ACV' phenotype. A distinctive feature of this mutant is the high level of resistance exhibited to ACV (100 pM) and the concomitant presence of a functional TK activity. Such a property makes this virus useful as a model for the study of viral resistance to nucleoside-type analogues in HSV.

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