Purification and characterization of two
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Anu Wallecha; Saroj Mishra
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Article
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2003
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Elsevier Science
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English
โ 265 KB
The thermo-tolerant yeast Pichia etchellsii produced two cell-wall-bound inducible beta-glucosidases, BGLI (molecular mass 186 kDa) and BGLII (molecular mass 340 kDa), which were purified by a simple, three-step method, comprising ammonium sulfate precipitation, ion-exchange and hydroxyapatite chrom