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Pregnenolone metabolism in rodent embryonic neurons and astrocytes

✍ Scribed by Khadija Kabbadj; Martine El-Etr; Etienne-Emile Baulieu; Dr. Paul Robel

Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
544 KB
Volume
7
Category
Article
ISSN
0894-1491

No coin nor oath required. For personal study only.

✦ Synopsis

The rat CNS has been previously shown to synthesize pregnenolone (PREG) and to convert it into progesterone (PROG) and some of its 5.c~-reduced metabolites. However, the brain cell types involved in the metabolic conversions of PREG are poorly known. Selective conditions were used to obtain purified cultures of neurons and astrocytes from mouse or rat fetal striatum and cerebral cortex. Neurons converted PREG to only one identified metabolite, 20a-dihydro PREG, whereas astrocytes converted PREG also to PROG, 6a-dihydro PROG, and 3a (36)-5a-tetrahydro PROG. Therefore, astrocytes can convert the neurosteroid PREG into the steroid hormone PROG and the neuromodulatory steroid 3u, 5w-tetrahydro PROG, whereas neurons lack the 115-3phydroxysteroid dehydrogenase isomerase activity (and cholesterol side-chain cleavage activity), necessary for the biosynthesis of PROG. Provision of steroid substrates is another example of cross-talk between glial cells and neurons. Q 1993 Wlley-Llss, Inc

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