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Pregnancy rate and survival in culture of bovine IVF embryos frozen in various cryoprotectants and thawed in a one step system

โœ Scribed by T. Suzuki; M. Takagi; M. Yamamoto; S. Saha; A. Boediono; M. Oe


Book ID
118379684
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
74 KB
Volume
39
Category
Article
ISSN
0093-691X

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Eight-cell mouse embryos were frozen by using glycerol, ethylene glycol, or erythritol as the cryoprotectant. The samples were slowly cooled (0.5"C/min) to temperatures between -10 and -79ยฐC before direct transfer to liquid nitrogen (-196ยฐC) and thawed slowly ( -20"C/min) or rapidly ( -500ยฐC/min). T