Pregnancy-associated β1-macroglobulin: Detection on the surface of ovarian cancer cell lines

✍ Scribed by W. H. Stimson; A. McAdam; G. Lang; J. Dodd

• Book ID: 102278003
• Publisher: John Wiley and Sons
• Year: 1985
• Tongue: French
• Weight: 437 KB
• Volume: 35
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910350208

✦ Synopsis

Pregnancy-associated beta 1-macroglobulin (beta 1-PAM) is a serum protein which can be detected at elevated levels in the blood of many ovarian cancer patients. The presence of the antigen was investigated on the surface of 4 human ovarian cancer lines and 32 other human and rodent cell types and lines by means of immunofluorescent staining and anti-beta 1-PAM-enzyme conjugate. The antigen was detected only on the ovarian lines but no secretion of beta 1-PAM into the culture supernatants was observed. The ovarian cancer cells appeared to display the beta 1-PAM constantly during culture, and enzymic treatment of the cells indicated that detection of the cell-bound antigen with antiserum could be, at least partially, eliminated with proteases but not glycosidases or neuraminidase. The results serve to confirm the association of beta 1-PAM with ovarian cancer.