Preferential formation of deletions following in vivo exposure of postmeiotic Drosophila germ cells to the DNA etheno-adduct-forming carcinogen vinyl carbamate
✍ Scribed by Leo A. P. Ballering; Madeleine J. M. Nivard; Ekkehart W. Vogel
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 82 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0893-6692
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✦ Synopsis
DNA sequence changes induced in the vermilion VB, three carried large deletions. Twenty-two of 23 gene of Drosophila following in vivo treatment of base substitutions derived from either VCA or VB postmeiotic male germ cells with vinyl carbamate experiments fell into one of the four categories ex-(VCA), an etheno-adduct-forming carcinogen, are pected from e-adducts: three vermilion mutants had primarily deletions. With VCA, 65% (13/20) of the GC r AT transitions, five had AT r GC transitions, vermilion mutants isolated from crosses of NER / (nu-7 carried GC r TA transversions, and 7 were AT r cleotide excision repair) males with NER / females TA transversions. In view of the similarities in the and 40% (6/15) obtained from matings with NER 0 response of mouse and Drosophila germ lines to females were intra-or multi-locus deletions. Due to several classes of alkylating agents, a high incithe insufficiently low mutagenic activity in NER / ge-dence of deletions is predicted to occur as well in notypes of vinyl bromide (VB), another e-adductpostmeiotic germ cells of mice exposed to these forming carcinogen, vermilion mutants could only types of agents. Environ. Mol. Mutagen. 30:321be isolated from crosses of VB-treated males with 329, 1997 ᭧ 1997 Wiley-Liss, Inc. NER 0 females. Of 14 vermilion mutants induced by