Preferential expression of αEβ7 integrin (CD103) on CD8+ T cells in the psoriatic epidermis: regulation by interleukins 4 and 12 and transforming growth factor-β
✍ Scribed by Y. Teraki; T. Shiohara
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 351 KB
- Volume
- 147
- Category
- Article
- ISSN
- 0007-0963
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✦ Synopsis
Background:
Intraepidermal t lymphocytes are a critical element for sustaining the lesional pathology of psoriasis. integrin alphaebeta7 (cd103), a ligand for e-cadherin, may play a role in the localization of pathogenic t cells within the epidermis of psoriatic lesions. however, little information is available regarding alphaebeta7 expression on intraepidermal t cells in psoriasis.
Objectives:
To examine alphaebeta7 expression on intraepidermal t cells in psoriatic lesions and the regulation of alphaebeta7 expression on t cells in response to cytokines.
Methods:
T-cell expression of alphaebeta7 was examined by immunohistochemistry and flow cytometry. in vitro regulation of alphaebeta7 expression on cd4+ or cd8+ t cells purified from peripheral blood of healthy donors was also examined.
Results:
Immunohistochemical staining revealed expression of alphaebeta7 on a greater proportion of epidermal t cells than dermal t cells. nearly 30% of intraepidermal cd4+ t cells were found to express alphaebeta7 on flow cytometry, whereas more than 80% of intraepidermal cd8+ t cells expressed this integrin. in contrast, few t cells expressed alphaebeta7 in the peripheral blood of psoriatic patients. the in vitro culture experiment confirmed that alphaebeta7 was preferentially expressed on cd8+ t cells after stimulation with anti-cd3 monoclonal antibodies. addition of transforming growth factor-beta and interleukin-4 upregulated alphaebeta7 expression on t cells, whereas interleukin 12 downregulated this. furthermore, alphaebeta7 expression on established memory cd8+ t cells was not so reversible as that on cd4+ t cells.
Conclusions:
Preferential and stable expression of alphaebeta7 on cd8+ t cells may be involved in the lesional pathology of psoriasis.