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Preferential association of uracil-DNA glycosylase activity with replicating SV40 minichromosomes

โœ Scribed by Hans Krokan

Book ID
115912236
Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
225 KB
Volume
133
Category
Article
ISSN
0014-5793

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Proteins bound to SV 40 DNA in sarkosyl-treated nuclei have been studied. The major component is topoisomerase I, a 60-70 kDa protein which possesses a strong DNA-nicking activity in the presence of sarkosyl and camptothecin. An SV 40 DNA fraction containing sarkosyl-resistant proteins constitutes 2