Body composition data estimated for normal young, normal adult, and adult obese Wistar rats by a tritiated water (THO) dilution procedure (b. wt. range 119-237 g, n=31; 305-597 g, n=43; 274-381 g, n=6) were compared with the results of whole body analysis. As for the predicted total body lipid FBL)
Prediction of total body lipid from total body water in rats. Part 1. Relations between directly measured major body components
โ Scribed by Zahn, L. ;Freund, R. ;Hoppe, K. ;Pisarchuk, K. L. ;Augustin, W.
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 546 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0027-769X
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โฆ Synopsis
Aimed to the construction of a prediction equation for estimations of lipid content from animal water content body composition was determined by whole body analysis of male rats (1) given access, ad libitum, to a commercial standard diet (n = 144; ranging from 60 to 600 g in weight, and from the 4th to the 34th week of age), and (2) showing striking variations with regard to nutritional state, dietary history, enlarged fat deposition, genetic origin, intestinal microbial status, and advanced age (n = 75). It was shown that a unique coefficient of water content in lipid-free body mass does not exist.
The results of statistical analysis for the grouped values of percentage body dry matter (x) and percentage body lipid (y) indicate that the latter can be estimated accurately from body water content directly determined by the use of the quadratic regression equation y = -0.2864 x +0.01615 xz with a standard deviation of the procedure sy = k1.40. This prediction equation is valid for a wide developmental span even under highly different experimental states. Differences between the calculated body lipid contents vs. analytically determined values are smaller than by using a linear regression equation or coefficient(s) of hydration of lipid-free body mass.
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