Prediction of Protein Secondary Structure from Circular Dichroism Spectra: An Attempt to Solve the Problem of the Best-Fitting Reference Protein Subsets

✍ Scribed by B. Dalmas; W.H. Bannister

• Publisher: Elsevier Science
• Year: 1995
• Tongue: English
• Weight: 750 KB
• Volume: 225
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1995.1105

✦ Synopsis

In least-squares fitting of protein circular dichroism (CD) spectra using basis CD spectra for the respective secondary structure components, as given by reference proteins of known structural composition, good fits of the CD spectrum do not necessarily correspond to appropriate fits of the underlying structural composition of a test protein. In an attempt to overcome this problem, CD similarity measures were used to construct a subset of five reference (C D) spectra which permitted three-component fitting of the (\mathrm{CD}) and prediction of the relative magnitude of total (\beta)-sheet (antiparallel + parallel) and total other structure ( (\beta)-turn + remainder), relative to helix, in the test protein. A backpropagation neural network (BPN) was also trained to make this prediction. In subsequent five-component fitting of the CD spectrum, using a Monte Carlo method to generate subsets of reference proteins from the working data set, only those secondary structure fits which conformed to the consensus prediction of the CD similarity measures and the BPN were accepted. The method enhanced the fitting of antiparallel (\beta)-sheet and (\beta)-turn for (16 \mathrm{pro}-) teins, compared to the variable selection method of (P). Manavalan and W. C. Johnson (1987, Anal. Biochem. 167, 76-85). Some other proteins were less well fitted. Improvement in results is expected with a larger representation of feasible basis CD spectra in the working reference proteins. & 1995 Academic Press, Inc.