✦ LIBER ✦

Prediction of peptide retention times

✍ Scribed by Yasuhiro Sakamoto; Nami Kawakami; Tatsuru Sasagawa

Book ID: 104144694
Publisher: Elsevier Science
Year: 1988
Tongue: English
Weight: 589 KB
Volume: 442
Category: Article
ISSN: 1873-3778
DOI: 10.1016/s0021-9673(00)94457-1

No coin nor oath required. For personal study only.

✦ Synopsis

A new approach for predicting the retention times of peptides, either with isocratic or gradient elution is described. The isocratic capacity factors of peptides are correlated with their molecular weights and with their hydrophobicities. Given the experimental conditions, and the amino acid composition, it is possible to calculate the retention time of a peptide eluted by a gradient, for any slope of gradient, flow-rate and column length.

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