## Abstract Hydrophobic interaction chromatography (HIC) is an important technique for the purification of proteins. In this paper, we review three different approaches for predicting protein retention time in HIC, based either on a protein's structure or on its aminoβacidic composition, and we hav
β¦ LIBER β¦
Predicting Protein Retention Time in Hydrophobic Interaction Chromatography
β Scribed by M. E. Lienqueo; A. Mahn
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 234 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0930-7516
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## Abstract Hydrophobic interaction chromatography (HIC) uses weakly hydrophobic resins and requires a saltingβout salt to promote proteinβresin interaction. The saltingβout effects increase with protein and salt concentration. Dynamic binding capacity (DBC) is dependent on the binding constant, as