The classical disruption method for yeast genes is by using in vitro deletion of the gene of interest, or of a part of it, with restriction enzymes. We are now routinely using a strategy that takes advantage of polymerase chain reactions (PCRs) which amplify large pieces of DNA. Since this approach
Predicting functional upstream open reading frames inSaccharomyces cerevisiae
β Scribed by Selpi; Christopher H Bryant; Graham JL Kemp; Janeli Sarv; Erik Kristiansson; Per Sunnerhagen
- Book ID
- 114998867
- Publisher
- BioMed Central
- Year
- 2009
- Tongue
- English
- Weight
- 310 KB
- Volume
- 10
- Category
- Article
- ISSN
- 1471-2105
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Open reading frames longer than 300 bases were observed in the antisense strands of the genes coding for the glycolytic enzymes phosphoglucose isomerase, phosphoglycerate mutase, pyruvate kinase and alcohol dehydrogenase I. The open reading frames on both strands are in codon register. It has been s
Conserved upstream open reading frames (uORFs) are found within many eukaryotic transcripts and are known to regulate protein translation. Evidence from genetic and bioinformatic studies implicates disturbed uORF-mediated translational control in the etiology of human diseases. A genetic mouse model