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Precise mapping and molecular characterization of the MFT1 gene involved in import of a fusion protein into mitochondria in Saccharomyces cerevisiae

✍ Scribed by Masaki Ito; Akira Yasui; Atsushi Komamine


Book ID
115927273
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
776 KB
Volume
320
Category
Article
ISSN
0014-5793

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The cloned DNA polymerase I gene has been used to map the POL1 locus on the left arm of chromosome XIV, between MET4 and TOP2. Temperature-sensitive mutants in POL1 have been obtained by in vitro mutagenesis of the cloned gene and in vivo replacement of the wild-type allele with the mutated copy. Ph