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Pre-column derivatization of free bile acids for high-performance liquid chromatographic and gas chromatographic—mass spectrometric analysis

✍ Scribed by Humberto E.C.S. Ferreira; William H. Elliott


Book ID
107996896
Publisher
Elsevier Science
Year
1991
Weight
602 KB
Volume
562
Category
Article
ISSN
0378-4347

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## Reversed-phase high-performance liquid chromatography (RP-HPLC) is a powerful method for assaying physiological amino acid concentrations in biological fluids. Four pre-column derivatization methods, with o-phthaldialdehyde (OPA), 9fluorenylmethyl chloroformate (FMOC-Cl), phenyl isothiocyanate