Pre-coating of microbial cells by hydrophobic reagents on immobilization

✍ Scribed by M. Kumakura; I. Kaetsu

• Book ID: 104634424
• Publisher: Springer Netherlands
• Year: 1983
• Tongue: English
• Weight: 247 KB
• Volume: 5
• Category: Article
• ISSN: 0141-5492
• DOI: 10.1007/bf00131902

✦ Synopsis

Strcptomyces phaeochromogenes cells were immobilized in a polymer matrix of 2-hydroxyethyl methacylate (HEPA) by irradiation-induced polymerization of the monomer frozen with the cells. The matrix with entrapped cells was cut into flakes. Activity of glucose isomerase in the matrix was 70-308 of that in non-immobilized cells, and the Michaelis constant was similar (0.36-0.3OM). Leakacje of cells from the immobilized matrix was prevented by coating the cells with hydrophobic monomers (e.9. tetraethyleneglycol acrylate) prior to immobilization in the FIEMA Folymer matrix.