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Practical limits of resolution in confocal and non-linear microscopy

✍ Scribed by Guy Cox; Colin J.R. Sheppard

Publisher
John Wiley and Sons
Year
2003
Tongue
English
Weight
154 KB
Volume
63
Category
Article
ISSN
1059-910X

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✦ Synopsis

Abstract

Calculated and measured resolution figures are presented for confocal microscopes with different pinhole sizes and for nonlinear (2‐photon and second harmonic) microscopes. A modest degree of super‐resolution is predicted for a confocal microscope but in practice this is not achievable and confocal fluorescence gives little resolution improvement over widefield. However, practical non‐linear microscopes do approach their theoretical resolution and therefore show no resolution disadvantage relative to confocal microscopes in spite of the longer excitation wavelength. Microsc. Res. Tech. 63:18–22, 2004. © 2003 Wiley‐Liss, Inc.

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